Friday, February 13, 2026

History of Microbiology Application in Nepal: “10 Unknown facts”

 Microbiology may seem like a science reserved for laboratories and scientists, but in Nepal, it’s a fundamental part of daily life—embedded in traditions, food, health remedies, and even farming practices. At its core, microbiology explores the invisible realm of microorganisms—bacteria, viruses, fungi, and protozoa—that play a vital role in shaping our environments and health. While Louis Pasteur is known as the father of microbiology for his groundbreaking discoveries, countless Nepalese have been harnessing the power of microbes for centuries, often without realizing the science behind their customs. 

One classic example is the tradition of turning milk into curd, a staple in Nepalese households. By simply adding a spoonful of previous curd to fresh milk, families rely on beneficial bacteria to thicken the milk over several hours, creating a nutritious food that aids digestion and boosts gut health. This simple act of fermentation not only preserves milk but also enhances its nutritional value—a perfect synergy of tradition and science. 


                                        Fig: Curd preparation using curd itself as starter culture 

Another age-old practice is the making of Gundruk, a beloved fermented dish made from leafy greens such as mustard or radish leaves. Villagers pack greens into containers and allow natural bacteria to ferment them, resulting in a tangy, flavorful food rich in probiotics. Gundruk is especially important in rural diets, providing essential nutrients during lean seasons and contributing to a healthy digestive system.


                                                        Fig: Gundruk 

Bamboo shoots, known as Tama, undergo a similar transformation. Through fermentation, potentially harmful compounds are broken down, and the shoots become both safe and delicious. This process is a testament to how microbial action can turn raw, sometimes inedible materials into cherished delicacies, all while enhancing their shelf life. 


                                           Fig: Tama prepared from Bamboo shoot

 Even in agriculture, microbiology plays a quiet but crucial role. When processing jute plants, farmers submerge them in water, allowing natural microbes to break down the tough fibers. This process, called retting, makes the fibers pliable for weaving into ropes and mats. The understanding of timing and water conditions, passed down through generations, shows an intuitive grasp of microbial processes.


                                          Fig: Jute plant for biodegradation under water 

In beverage-making, the traditional drink Chayang is brewed from grains mixed with marcha, a starter culture rich in the yeast Saccharomyces cerevisiae. The yeast fuels fermentation, transforming starches into alcohol and giving the drink its distinctive taste. This homemade brewing process not only provides a source of social enjoyment but also exemplifies a deep rooted knowledge of fermentation.


                                         Fig: Prepared Chayang using grains and marcha 

Folk remedies in Nepal often draw upon microbial principles as well. For instance, egg whites are sometimes used to cover wounds, creating a natural, protective barrier that helps prevent bacterial infection and promotes healing. While modern medicine offers advanced solutions, these traditional approaches are still valued in rural areas.


                                                 Fig : Wound Treatment using egg white

 Jute plants continue to be useful even after harvest. The leftover plant matter is plowed back into the fields, enriching the soil as it decomposes. This practice harnesses the power of soil microbes to recycle nutrients, enhancing soil fertility and supporting sustainable agriculture—a vital strategy for smallholder farmers.


                                                     Fig: Jute plant in field 

Food preservation is another area where microbiology quietly works its magic. Salt is widely used to make pickles, such as tangy mango achar. The high salt concentration inhibits the growth of spoilage-causing microbes while allowing beneficial bacteria to flourish, ensuring the pickles last for months without refrigeration. 


                                                   Fig: Mango Pickle Preserved using salt

High in the Himalayan mountains, communities collect Yarshagumba, a rare fungus known scientifically as Ophiocordyceps sinensis. Revered as a natural remedy, it’s believed to strengthen immunity and vitality. While modern science is still unraveling its health benefits, generations of Nepalese have trusted in its restorative powers, showcasing the intersection of traditional knowledge and microbiology.


                                                      Fig: Yarshagumba

 Lastly, the herb Chirata (Swertia chirayita) is used to treat skin diseases, thanks to its antimicrobial properties. Local healers have long applied Chirata extracts to skin ailments, relying on the plant’s ability to inhibit harmful microbes and promote healing—a testament to the wisdom embedded in folk medicine.

                                                   Fig: Use of chirata for skin disorder 

These examples reveal that microbiology in Nepal is not confined to academic textbooks or research labs. Instead, it is woven into the fabric of everyday living—found in kitchens, fields, and medicine chests. By intuitively using microorganisms, Nepalese people have developed ingenious ways to improve health, preserve food, and sustain their environment, proving that the unseen world of microbes has a very visible impact on life.

How to protect From Urinary tract infection: Home Care and Lifestyle Tips


  Introduction

A urinary tract infection (UTI) is an infection in any part of the urinary system, which includes the kidneys, ureters, bladder, and urethra. Most infections involve the lower urinary tract— specifically the bladder (cystitis) and the urethra (urethritis). UTIs are common, especially in women, and are typically caused by bacteria like E. coli entering the urethra.

 

 Causative Agents

The primary causative agent for Urinary tract infection (UTIs) is bacteria, most commanly Escherichia coli (E.coli),and other bacteria  like Klebsella pneumonae, proteus miralibis and Enterococcus. Common fungi involved in UTI Include Candida albicans.Organism involved in UTIs  generally originates from the gut and enter the urinary tract.

    Escherichia coli (E.coli) is the most common which originates from gut and accounts for 75-95% of cases world wide.

                                               

                                        Figure: E.Coli

 

Symptoms

1. Lower UTI: Lower abdominal pain, Cloudy/blood in urine, sudden urge to urinate etc.

           2. Upper UTI :High body temperature (above 100.4°F, Chills, Confusion, Pain in back


 Diagnosis

·    Urine analysis: A "clean-catch" midstream urine sample is collected in sterile container and tested for white blood cells (leukocytes), red blood cells (hematuria), and nitrites (produced by certain bacteria like E.coli ) and urine culture.

 

 



                                          Figure: RBC/WBC count by Microscopy

·    Urine Culture: 

Urine culture is considered as gold standard test that specifies type of bacteia involved in infection and also which antibiotic are most effective through Antibiotic sensitive testing.



                                 Figure: organism from urine on culture media


                                  Figure: Antibiotic sensitivity test of organism 


 Treatment

1. Simple (Uncomplicated) UTIs: Typically affect healthy, non-pregnant women. Common medications include:

Ø Nitrofurantoin: Often prescribed for 5 days.

Ø Trimethoprim-sulfamethoxazole: Commonly taken for 3 days.

Ø Fosfomycin : A single-dose powder mixed with water.

Ø Cephalexin or Cefdinir: Often used for 5 to 7 days.

 

2. Complicated or Severe Infections: If the infection spreads to the kidneys (pyelonephritis) or occurs in men or pregnant women, longer courses or different medications is required.

Ø Fluoroquinolones (Ciprofloxacin, Levofloxacin): Reserved for complicated cases or when other options are unsuitable due to the risk of serious side effects.

Ø Intravenous (IV) Antibiotics: Required in case of severe infections that cause vomiting, high fever, or sepsis, often requiring a hospital stay.

 Home Care and Lifestyle


  1. Hydration and Urinary Habits Drink enough water—aim for about 6 to 8 glasses, or 1.5 to 2 liters a day. This keeps your urine diluted and helps flush out bacteria hanging around your urethra. Don’t ignore the urge to pee. Waiting too long just gives bacteria more time to multiply. When you do go, take your time and empty your bladder fully. Leaving a little behind gives bacteria a chance to stick around. Hygiene Practices Always wipe from front to back, especially for women. This simple habit keeps bacteria from the anus away from your urethra. Stick to showers over baths. Long soaks, hot tubs, or bubble baths can push bacteria where you really don’t want it. Be picky about products—avoid feminine sprays, douches, or anything scented around your genitals. These can irritate the area. And if you’re on your period, change tampons or pads regularly. It keeps bacteria from growing. Sexual Health and Hygiene Pee right after sex. It’s a quick way to flush out any bacteria that might have wandered in during the action. If things get dry, use a water-based lubricant. It keeps irritation down and protects your urethra. When it comes to birth control, skip diaphragms or spermicidal gelsthey mess with your natural bacterial balance and can make UTIs more likely. Clothing and Lifestyle Go for cotton underwear. It breathes, keeps things dry, and bacteria don’t thrive as easily. Avoid tight pants or synthetic fabrics—they trap heat and moisture. And don’t let yourself get constipated. A high-fiber diet keeps things moving and helps prevent bacterial buildup. Natural Remedies and Supplements Try unsweetened cranberry juice or cranberry supplements. They’ve got compounds that help keep bacteria from sticking to your bladder. Add some probiotics to your diet—yogurt with live cultures, for example—to support healthy bacteria. D-mannose, a natural sugar found in some fruits, can also stop bacteria from sticking around. And don’t forget vitamin C. It makes your urine more acidic, which helps keep bacteria in check.




 






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